A1594 Urinary profiles of monohydroxylated metabolites of polycyclic aromatic hydrocarbons in different industrial activities

Monday, March 19, 2012: 16:20
Costa Maya 1 (Cancun Center)
Catherine Nisse, University department of occupational health, Université Lille, Lille Cedex, France
Nadège Lepage, Occupational Health Services, CHRU Lille, Lille, France
Ariane Leroyer, University department of occupational health, Université Lille, Lille, France
Mickael Howsam, University Centre of Measurements and Analysis, Université Lille, Lille, France
Patrice Simon, Pollutants and Health, INRS (National Institute of Resaearch and Security), Nancy, France
Anne Maitre, Occupational and Environmental Toxicology Laboratory, CHU de Grenoble, Grenoble, France
Jean-paul Bonte, University Centre of Measurements and Analysis, Université Lille, Lille, France
Michel Lhermitte, Toxicology and genopathy laboratory, CHRU Lille, Lille, France
Handouts
  • HAP_nisse_19031_def.pdf (1.1 MB)
    • Introduction
    The aim of the study was to assess the profile of exposure to polycyclic aromatic hydrocarbons (PAHs) of workers employed in different industrial activities by analysing the excretion of 15 PAHs monohydroxylated metabolites and by the measure of atmospheric concentrations of 16 PAHs.

    Methods
    The study population consisted of 125 male workers (age: 21-57, mean: 41 years), employed in aluminium production (n= 20), coke production (n= 45), glassware production (n= 15), landscape service activities (n= 15), machining of metals (n= 30). External PAHs exposure was assessed by personal air sampling of 16 PAHs. Internal PAHs exposure was determined by the quantification of urinary PAHs metabolites (using HPLC-MS/MS and HPLC-Fluorimetry): 2-,3-&1-,4-,9-hydroxyphenanthrenes (S-OH-phen), 1-hydroxypyrene (1-OH-pyr), 3-hydroxybenzo(a)pyrene (3-OH-B(a)P), 1-&2-hydroxynaphalenes (S-OH-naph), 2-hydroxyfluorene (2-OH-fluo), 3-hydroxyfluoranthene (3-OH-flua), 1-&2- hydroxybenzo(a)anthracenes (S-OH-B(a)A), 3-&6-hydroxychrysenes (S-OH-chry), measured in post-shift urine samples of all workers and 16 hours later.

    Results
    Median total PAHs atmospheric concentrations were respectively: 12929, 118314, 1157, 17479, 365 microg.m-3 in aluminium production, coke production, glassware production, landscape service activities, machining of metals. At the end of the shift, in the total study population, the frequency of detection was respectively of 100% for 1-OH-pyr, 1-&2-OH-naph, 2-,3-OH-phen, 1-,4,-9-OH-phen, 3-OH-flua, 90% for 2-OH-fluo, 71% for 3-OH-B(a)P. Other metabolites were not detected. Looking at urinary metabolites PAHs excretion profiles, variations were observed between industrial activities, S-OH-naph being always the major metabolites excreted. The highest median of 1-OH-pyr and 2-,3-OH-phen appeared in aluminium production, while all others metabolites were maximal in coke production. Significant correlations were observed between the different metabolites quantified and the sum of 16 PAHs atmospheric concentrations, the better correlation being with 1-OH-pyr (p<10-4) and the weakest with 3-OH-flua (p=0.06).

    Discussion
    This study provides information on actual levels of exposure to different PAH in several industrial activities and on the interest of using other metabolites than the well-established marker 1-hydroxypyrene. 

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